In recent years, mRNA vaccines and drugs have received widespread attention around the world. Areterna LLC is proud to introduce Fluc-eGFP mRNA (Catalog number: 11013-CAP), a cutting-edge tool to accelerate your mRNA research. This mRNA encodes both eGFP and luciferase, facilitating dual-reporter assays and transfection efficiency evaluations.
As shown in Figure 1, the optimized design features a high proportion (>95%) of the Cap1 cap structure for enhanced stability and expression. The sequence is optimized with codons, UTR, and polyA structures suitable for mammalian cells. Additionally, N1-Me-pUTP is used to replace natural UTP in the sequence, resulting in lower immunogenicity and better stability in vivo, thereby achieving higher expression efficiency.
Experimental Data Explained
- mRNA Integrity Detection
Q-sep100 analysis confirms a higher than 90% Fluc-eGFP mRNA integrity, ensuring its functionality and stability.
Capping Efficiency
LC-MS reveals a remarkable capping efficiency of Fluc-eGFP mRNA >95%, guaranteeing efficient translation initiation.
- mRNA Activity Detection
24 hours after transfection, eGFP and luciferase fluorescence activity were readily detectable in mRNA.
Key Product Advantages
- Dual-Reporter Capability
Fluc-eGFP mRNA is translated in vivo, allowing for Peptide 2A-mediated cleavage by endogenous proteases. This enables independent expression of eGFP and luciferase, which can be used to evaluate the transfection efficiency and delivery effectiveness of transfection reagents at the cellular level and animal level.
- Versatile Detection
Fluc-eGFP mRNA expression can be conveniently monitored by eGFP fluorescence microscopy or luciferase reporter assays, providing accurate and versatile data for diverse research applications.
Conclusion
Fluc-eGFP mRNA (Catalog number: 11013-CAP) from Areterna can empower research with its exceptional quality, versatility, and convenience. The dual-report functionality mRNA is an ideal tool for transfection efficiency and delivery system evaluations.
Related Products
| SKU | Description |
| 11013-CAP-1 | Fluc-eGFP mRNA with N1-Me-pUTP(5’CAP), 1mg |
| 11013-CAP-5 | Fluc-eGFP mRNA with N1-Me-pUTP(5’CAP), 5mg |
| 11013-CAP-10 | Fluc-eGFP mRNA with N1-Me-pUTP(5’CAP), 10mg |