CAP4 GAG (UNA) and CAP5 GAG (ENE)
CAP4 GAG (UNA) and CAP5 GAG (ENE) Cap Analogs
CAP4 and CAP5 are variations of m7GpppAmG trinucleotide, the first generation of the cap analog. The innovation of CAP 4 and CAP 5 is on the m7G cap, with CAP4 having a UNA with a unlocked nucleic acid structure and CAP5 having a double bond between the ribose ring and the first phosphate group.
Technical Data
IVT Performance Comparison of Novel Cap Analogs
IVT Reaction System
The table shows the manufacturing efficiency of CAP GAG, a type of mRNA cap analog, by two different manufacturers: Company A and Areterna LLC. The table includes data on mRNA yield, mRNA integrity, capping efficiency, and dsRNA ratio.
| Cap Analog | Vendor | mRNA Yield (ug) | mRNA Integrity | Capping Efficiency | dsRNA Ratio |
| CAP GAG | Company A | 183 | 90.2% | 98.5% | 0.04% |
| CAP GAG (UNA) | Areterna | 187 | 91.3% | 99.1% | 0.03% |
| CAP GAG (ENE) | 184 | 89.5% | 98.9% | 0.04% |
Decapping Enzyme Performance Comparison
Decapping Reaction System
This table shows the results of a decapping reaction system on RNA with a 5′ cap, using a decapping enzyme with different cap analogs.
| Cap Analog |
Vendor | Remaining Cap% (Replicate 1) | Remaining Cap% (Replicate 2) | Remaining Cap% (Replicate 3) |
| CAP GAG | Company A | 22.5% | 19.3% | 28.4% |
| CAP GAG (UNA) | Areterna | 77.3% | 82.5% | 84.0% |
| CAP GAG (ENE) | 35.9% | 35.2% | 34.4% |
In-Vivo Performance of mRNA’s Capped with Novel Cap Analogs
The image shows the expression of GFP from mRNA constructs with different CAP’s. The images were taken at 6, 24, 48, 72, and 96 hours after transfection.
This image shows luciferase protein expression from mRNA constructs with different CAP’s. The mice were monitored for 6, 24, 48, and 72 hours.
Product Details
CAP 4 m7G(UNA)(5')ppp(5')(2'OMeA)pG 100mM Ammonium Solution
| SKU | Size | Purity: >98% |
| CAP4011-0.1 | 100μL | |
| CAP4011-1 | 1mL | |
| CAP4011-10 | 10mL | |
| CAP4011-100 | 100mL | |
| CAP4011-500 | 500mL |
CAP 5 m7G(5')vppp(5')(2'OMeA)pG 100mM Ammonium Solution
| SKU | Size | Purity: >98% |
| CAP5011-0.1 | 100μL | |
| CAP5011-1 | 1mL | |
| CAP5011-10 | 10mL | |
| CAP5011-100 | 100mL | |
| CAP5011-500 | 500mL |