T7 RNA polymerase (low dsRNA)
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T7 RNA polymerase (low dsRNA)
| Category | mRNAs: IVT Enzyme |
| Product Name | T7 RNA polymerase (low dsRNA) |
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Product Description
This enzyme is a mutant form of T7 RNA polymerase derived from E. coli expression intended for use in in-vitro transcription (IVT) to generate mRNA. It utilizes double-stranded DNA with the T7 promoter sequence (5’-TAATACGACTCACTATA-3’) as a template and NTPs or modified NTPs as substrate. Either linearized plasmids or PCR products can be used as IVT templates. Compared to the wild-type T7 RNA polymerase, this product generates much lower level of double-strand RNA (dsRNA) during the IVT process, which makes the mRNA less immunogenic.
Lower dsRNA from IVT
Reduced immunogenicity due to lower level of dsRNA
GMP grade will be made available
Ordering Information
| Cat. No. | Size |
| 10308-20K | 100 μL/20KU |
| 10308-200K | 1 mL/200KU |
| 10308-2M | 10 mL/2MU |
Recommended IVT Condition
| Component | Volume |
| SYNTHGENE’s New CAP (100mM) | 2 μl |
| CTP / GTP/ ATP/ N1-Me-pUTP (100 mM each) | 2 μl each |
| T7 RNA Polymerase (low dsRNA) | 1 μl |
| 5×Transcription Buffer (low dsRNA) | 4 μl |
| Inorganic Pyrophosphatase (1 U/μL) | 0.04 μl |
| Murine RNase inhibitor (40 U/μL) | 1 μl |
| DNA Template | 1 μg |
| RNase free H2O | Up to 20 μl |